Clone Read LE_HBa0001E20_SP6_127309

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0001E20_SP6_127309
Primer
SP6
ID from Seq. Facility
Le-HBa001_E20-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
736 trimmed for vector and quality, 1336 raw
Genbank Accession
DU067959
Sequence
>LE_HBa0001E20_SP6_127309 (raw untrimmed)
GCCCCTATAGTTGCGCCCCATATTCTTTATATGTCGGGGTTGAAAAGAAGTGCAGTTAAATAAATTTGAGGAATAGGAAAAATATATTTTTTAAATTTTG
TATCATAACTTCAATAGCAGAGGTGAATAAAGTATTTGTTTTATATTCAGAAAAAAATAGATGAAATCTCACATATGTGTATTAAAATTTCAGAAATAGT
AGGATAATATATTCCAGAAAAAATTTTGGTAGCATCATAAAATGATTTCAAAAATATTCTAAGTTCATTTACTTCATCCCAATCACTATCATTAATTTTA
AATTCAGGATAAGCATTATGATTATTAAATAGCATAGTAATAGATTGTCTATATGCATAAGCAACTTCAAGCATATCATAAAAGGAATTCCATCTAGTTT
TTACATGCTTCGGAACTTTTCTAAACGGAAGTTTAAATGCATTACAAAGTTCTTTAAATTGATTAATTCTACTACTACTATTCATATGAAAAATGTAAAA
ACAAGCATTATCGATTTTATCACAACTATTTTCAAATAATTTCACACCGTCACTAACAACCAAATTTAATATATGCGCGGCACATCTAACATGAAAAGCA
TATTTACTAATAGGACAAAGTCTAGGTTCTAGCATATTAATAGCATTTAAATTAGCAGAGGCATTATCTAAAGTAATACTAACGACTTTATCACAAAGAC
CAAAAAAATCTAAAATTTCCAACACAGTAGTAGCAATATAAATTTCGATTTTTTTCTTTTGACAAATTTTATAACCAATAATTCTTTTTTGTAAATTCCA
ATTATAATCGATCCAATGCGCAGTAACAGTTAAATAATCAAAACCATTAGGACTACAACCCATATCAGTAGTAATAGCACCTCTACAATCCATTAATTTC
AAATAGGCACGTAAATTATTGACATGTTTTTTTTTGAAAATTCAAAAATATCTTTACCATGCCTTCTTGAAAAACCTTTAAAAATAGGAATATTTTGTTT
CCACAAAATTAAGCAATAAAACCCGGAATGTTCCCCAAAAAAAAAAAGGGAAAACCACAAAACAACAACCCTTTTTAAAAAAATTTTTTCCCCATCCCCA
ATTCTTTGTTTTAGGTTTTTAATGTGGAAAAAGGGACCCCTTGGGGGTTTAAAGATTTTATAACTCCCATTTTGAAACCCAATATTTTAAACTCCCCTCA
ACCCATATTTTTTTACAATTTCAAATTTTCTCCAACAATTTTCAAAAAAAAAAAAAATATTATTCCAAAACCCCCCCCTTTTTACCATAGCCCCTATAAA
TAAAAACAAAAGATTTTTTTTTTTTTAAAACCCCCC
>LE_HBa0001E20_SP6_127309 (trimmed for quality and cloning vector)
CGGGGTTGAAAAGAAGTGCAGTTAAATAAATTTGAGGAATAGGAAAAATATATTTTTTAAATTTTGTATCATAACTTCAATAGCAGAGGTGAATAAAGTA
TTTGTTTTATATTCAGAAAAAAATAGATGAAATCTCACATATGTGTATTAAAATTTCAGAAATAGTAGGATAATATATTCCAGAAAAAATTTTGGTAGCA
TCATAAAATGATTTCAAAAATATTCTAAGTTCATTTACTTCATCCCAATCACTATCATTAATTTTAAATTCAGGATAAGCATTATGATTATTAAATAGCA
TAGTAATAGATTGTCTATATGCATAAGCAACTTCAAGCATATCATAAAAGGAATTCCATCTAGTTTTTACATGCTTCGGAACTTTTCTAAACGGAAGTTT
AAATGCATTACAAAGTTCTTTAAATTGATTAATTCTACTACTACTATTCATATGAAAAATGTAAAAACAAGCATTATCGATTTTATCACAACTATTTTCA
AATAATTTCACACCGTCACTAACAACCAAATTTAATATATGCGCGGCACATCTAACATGAAAAGCATATTTACTAATAGGACAAAGTCTAGGTTCTAGCA
TATTAATAGCATTTAAATTAGCAGAGGCATTATCTAAAGTAATACTAACGACTTTATCACAAAGACCAAAAAAATCTAAAATTTCCAACACAGTAGTAGC
AATATAAATTTCGATTTTTTTCTTTTGACAAATTTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.898107
Expected error %
0.1059
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_470386.1| Score 128 E Value 2e-28 Identity 11.10% Align. Length 701bp (95.24%) Frame 3
putative transposase [Oryza sativa (japonica cultivar-group)] gb|AAS07359.1| putative transposase [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_908365.1| Score 128 E Value 2e-28 Identity 11.10% Align. Length 701bp (95.24%) Frame 3
putative transposase [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0001E20
Clone
Clone name
hba-1e20
LE_HBa0001E20
P001E20
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
3 sequencing reads found