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Clone Read LE_HBa0195C20_SP6_126777

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0195C20_SP6_126777
Primer
SP6
ID from Seq. Facility
LE_HBa_195_C20-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
607 trimmed for vector and quality, 928 raw
Genbank Accession
DU054155
Sequence
>LE_HBa0195C20_SP6_126777 (raw untrimmed)
CCCAGGTTTTTTGAATAGTTTGGGGGATAATCCTGAAGGTTTATTGGGTTGATTCCAGGAACGCAAAGGTACTTGTCCTTTTAGCGCCTCCCCTGATTTA
GATCATCAGAACTGGTCCCTTCTAGAGGCACCATTGACTAACCAAATCTAATGCTTCCAGGTTGTTTCACCTTTGAGCGGACATATTGACTACTCATTTG
CGTCAGCATGGCATCCTGATGGCCTAACTTTTGCAACTGGCAACCAGGATAAAACCTGCAGAGTATGGGATATACGGAACCTGTCTAAATCAGTTGTTGC
TCTGAAGGGTAACCTTGGAGCTATTCGGTCAATCCGCTACACATCTGATGGAAGATTCATGGCAATGGCAGAACCTGCAGATTTCGTTCATGTTTTCGAT
GTAAAAAATGGTTTTTATCAGGAGCAAGAAATTGATTTCTTTGGTGAGATATCAGGTCTGTCTTTTAGTCCTGATACAGAGTCCCTTTTTGTCGGAGTCT
GGGATCGCACATATGGTAGCCTTCTTGAGTTTGGACGGCGGCACAACTACTCATACCTTGATACAATTATGTAAACACTCTCTTGAATGCTGCTTTGGGT
ATGTTTACTTGGCTTGAAGTGGCGCTGGCTGAACTTTGAAAAACGAGTAGCAAATTCAGGAGTTAGTAGTGGATTTGGTTGTAAATTGGCCATGTTGCGC
AAATTTAAATTTGGCACATAAATTGTTGTGGCCCATTACTATATATCAAGTATGTTAATTGGAGGAAGTGTATACAGTAGGGGAAACTCCGTGGTGTGGA
GCTGAAAAAGTTCTTGTTTTCTTATGAAATCCCTAGTATTGCTCTCGTTTAGTGTGGGGGGGTTATCTCCTCTATTTGAAACCTCGATCACATATTCATG
TTTTTCTTTTTTTTTTTTTTTTTTTTTN
>LE_HBa0195C20_SP6_126777 (trimmed for quality and cloning vector)
ATCCTGAAGGTTTATTGGGTTGATTCCAGGAACGCAAAGGTACTTGTCCTTTTAGCGCCTCCCCTGATTTAGATCATCAGAACTGGTCCCTTCTAGAGGC
ACCATTGACTAACCAAATCTAATGCTTCCAGGTTGTTTCACCTTTGAGCGGACATATTGACTACTCATTTGCGTCAGCATGGCATCCTGATGGCCTAACT
TTTGCAACTGGCAACCAGGATAAAACCTGCAGAGTATGGGATATACGGAACCTGTCTAAATCAGTTGTTGCTCTGAAGGGTAACCTTGGAGCTATTCGGT
CAATCCGCTACACATCTGATGGAAGATTCATGGCAATGGCAGAACCTGCAGATTTCGTTCATGTTTTCGATGTAAAAAATGGTTTTTATCAGGAGCAAGA
AATTGATTTCTTTGGTGAGATATCAGGTCTGTCTTTTAGTCCTGATACAGAGTCCCTTTTTGTCGGAGTCTGGGATCGCACATATGGTAGCCTTCTTGAG
TTTGGACGGCGGCACAACTACTCATACCTTGATACAATTATGTAAACACTCTCTTGAATGCTGCTTTGGGTATGTTTACTTGGCTTGAAGTGGCGCTGGC
TGAACTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.971511
Expected error %
0.9838
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g55680.1 Score 246 E Value 5e-66 Identity 25.20% Align. Length 455bp (74.96%) Frame 1
68414.m06374 WD-40 repeat family protein contains 2 (1 significant) WD-40 repeats (PF0400); similar to Trp-Asp repeat protein (PIR:T40094) [Schizosaccharomyces]
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g13340.1 Score 246 E Value 5e-66 Identity 27.10% Align. Length 413bp (68.04%) Frame 1
68416.m01679 WD-40 repeat family protein contains Pfam profile: PF00400 WD domain, G-beta repeat (3 copies, 1 significant); similar to Trp-Asp repeat protein (PIR:T40094) [Schizosaccharomyces]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAL47499.1| Score 246 E Value 2e-64 Identity 25.20% Align. Length 455bp (74.96%) Frame 1
unknown protein [Arabidopsis thaliana] gb|AAK25840.1| unknown protein [Arabidopsis thaliana] ref|NP_564699.1| WD-40 repeat family protein [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF79507.1| Score 246 E Value 2e-64 Identity 25.20% Align. Length 455bp (74.96%) Frame 1
F20N2.10 [Arabidopsis thaliana] pir||A96599 protein F20N2.10 [imported] - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0195C20
Clone
Clone name
hba-195c20
LE_HBa0195C20
P195C20
Clone type
BAC
Contamination
none
Estimated length
80,863
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found