Clone Read LE_HBa0163O03_T7_123593

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0163O03_T7_123593
Primer
T7
ID from Seq. Facility
LE_HBa_163_O03-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
604 trimmed for vector and quality, 849 raw
Sequence
>LE_HBa0163O03_T7_123593 (raw untrimmed)
TCAACCTCGGACCCGGGGGTATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTGCTCGTTTTATGGATCCCCTTTAAGACCCCGTTACCACATCTCTT
GATAGTAATGAAGCTTTTGAAAACCTGGGAGCATTGAGGGCTATTGACGAGCTAATAGATGTCACCATCAGCGAAAATGCATCAAAAGTGGCAAAATTCT
CCAACTACATGCGCGTTGCTTTTGAAACAAAGCGTGATCCTGAAATCTTGGTCCTTGCTAGTAAAGTTCTTGGTCACCTAGCTAGATCTGGTGGTGCAAT
GACTGCAGATGAAGTGGAACGTCAGGTGTGTAACATTGTGATACTTCAGAGACTTGATTGTCTGACATCAGGATCTCAATTCTGTCATATTTTCAGGTAA
AAGTTGCATTAGAATGGCTCCGTGGTGAAAGAATTGAGTATCGTCGCTTTGCTGCTGTCTTAATATTGAAGGTATAATTAACATGTTTCTTCATGATTTG
TTGTATAAGAGGAAACTTTAAAGCTAGGTTTTATTTCTTTCATCCTTACATGATGAGAAAACTTTGATTTAAATTATAGATGCTATAAGACTTGACAGCT
AGAATTTTCTCAAAATTATTAGAATTGTATCCTAAAGGTTAACTTAGTGTTTTTTTTTCCAACACATGGCAATCCAATTAGGCAAACAAGAGACTAACAA
TGTCGATCCATTGGAAAAGTATAGATTACATTATACAATCGCTCTCTCTTAGGTGAAGACCATGGTATCTTTTCGCAATCCGTTTCTTCCCGCTGACGCC
GTCATAACGCCTGCACCTGGGAAACACTTCAAGTTCTGCATTCTATACG
>LE_HBa0163O03_T7_123593 (trimmed for quality and cloning vector)
AGCTTTTGCTCGTTTTATGGATCCCCTTTAAGACCCCGTTACCACATCTCTTGATAGTAATGAAGCTTTTGAAAACCTGGGAGCATTGAGGGCTATTGAC
GAGCTAATAGATGTCACCATCAGCGAAAATGCATCAAAAGTGGCAAAATTCTCCAACTACATGCGCGTTGCTTTTGAAACAAAGCGTGATCCTGAAATCT
TGGTCCTTGCTAGTAAAGTTCTTGGTCACCTAGCTAGATCTGGTGGTGCAATGACTGCAGATGAAGTGGAACGTCAGGTGTGTAACATTGTGATACTTCA
GAGACTTGATTGTCTGACATCAGGATCTCAATTCTGTCATATTTTCAGGTAAAAGTTGCATTAGAATGGCTCCGTGGTGAAAGAATTGAGTATCGTCGCT
TTGCTGCTGTCTTAATATTGAAGGTATAATTAACATGTTTCTTCATGATTTGTTGTATAAGAGGAAACTTTAAAGCTAGGTTTTATTTCTTTCATCCTTA
CATGATGAGAAAACTTTGATTTAAATTATAGATGCTATAAGACTTGACAGCTAGAATTTTCTCAAAATTATTAGAATTGTATCCTAAAGGTTAACTTAGT
GTTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.959715
Expected error %
0.8129
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g50030.1 Score 117 E Value 2e-34 Identity 61.30% Align. Length 421bp (69.70%) Frame 3
68414.m05614 target of rapamycin protein (TOR) identical to pTOR [Arabidopsis thaliana] GI:12002902; contains Pfam profiles PF00454 Phosphatidylinositol 3- and 4-kinase, PF02259 FAT domain, PF02260 FATC domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF76442.1| Score 117 E Value 1e-32 Identity 61.30% Align. Length 421bp (69.70%) Frame 3
Contains strong similarity to rapamycin associated protein FRAP2 from Homo sapiens gb|U88966 and contains a Phosphatidylinositol kinase PF|00454 domain. EST gb|W43444 comes from this gene. [Arabidopsis thaliana] pir||G96536 hypothetical protein F2J10.9 [imported] - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAW78347.1| Score 117 E Value 3e-33 Identity 64.50% Align. Length 421bp (69.70%) Frame 3
target of rapamycin [Zea mays]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0163O03
Clone
Clone name
hba-163o3
LE_HBa0163O03
P163O03
Clone type
BAC
Contamination
none
Estimated length
77,671
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found