Clone Read LE_HBa0151N15_SP6_123197

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0151N15_SP6_123197
Primer
SP6
ID from Seq. Facility
LE_HBa_151_N15-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
645 trimmed for vector and quality, 974 raw
Genbank Accession
DU054075
Sequence
>LE_HBa0151N15_SP6_123197 (raw untrimmed)
CGGGATTCCGCTTACTTCAGTTCCGGTATGTATATATTTGAATTATTTCGAGTGTGTTTGATATGATAGAAGTCATTTACTATTTAAAAAAAAAAGTGAA
AAAGAAACTATCGAATTACATTAATCATTCCTATCATATTTATTAATTTTCCTTATTATTTGAAATATTATCTCATTGATTAATAATTTCATACTAAATT
TCAAGTCAGATATACATACATGCGTGTTTTTTGTTACCAGATACACTTAAATAGAGCAAGAGACGAGTGAGATTTGTCTATATAACTAGTGCGATTCACA
TGTATGGGGGATATAAACCAGATACGAGAGCTATATAAGAGTCTTCCAGACACAATTTCTTTTTCTCATACCAAAAATACCCTCAATTTCTTTTTTTCAT
ACCAAAAATACCCTCAATTTTCTACATTCCAAATTAGACTAATGTTTTTTATTTTTTGGTCATAGCAAGCAATTGAAGAACTAGGGGTATATTTTGTATC
ATTTGATAGACCAGGTTATGGTAAAAGTGATCCTCATCCAAAGAGAAGTTTTAAAAGTTTAGCTCTTGATATTGAAGAAGTGGCTGATCAATTGGAACTT
GGAAATAAATTTTATGTTATTGGATTTGCAATGGGTGCACACTTTGTTTGGGGTTGCCTAAAGTACATACCTCATAGGTAACAACAATTTTTACTATTAC
TAATAAGGTCATTTTCCTGCTAAATTTGGTGATTTTTTGTGGTTTTACCAGGGTCGGATATCTGGTGGGGGGGTTTGATCAGTATAGATCTTGGGGTCGG
AAAGTTTATTTGGAGGAGATAAGGGGGTTGAATCTTCTGTTGTATCATTTAGTCTTTTGTAGGTCCAATTTTGAAAGCCACCGTTGTTACAGATTTCCTG
GAGACGACCCCCGTTGCGGGTGAAGAGTAACCGGGCTTTAGCGTGTTTGCGGCGACTAAAGGAGTACGATCGTC
>LE_HBa0151N15_SP6_123197 (trimmed for quality and cloning vector)
TATGTATATATTTGAATTATTTCGAGTGTGTTTGATATGATAGAAGTCATTTACTATTTAAAAAAAAAAGTGAAAAAGAAACTATCGAATTACATTAATC
ATTCCTATCATATTTATTAATTTTCCTTATTATTTGAAATATTATCTCATTGATTAATAATTTCATACTAAATTTCAAGTCAGATATACATACATGCGTG
TTTTTTGTTACCAGATACACTTAAATAGAGCAAGAGACGAGTGAGATTTGTCTATATAACTAGTGCGATTCACATGTATGGGGGATATAAACCAGATACG
AGAGCTATATAAGAGTCTTCCAGACACAATTTCTTTTTCTCATACCAAAAATACCCTCAATTTCTTTTTTTCATACCAAAAATACCCTCAATTTTCTACA
TTCCAAATTAGACTAATGTTTTTTATTTTTTGGTCATAGCAAGCAATTGAAGAACTAGGGGTATATTTTGTATCATTTGATAGACCAGGTTATGGTAAAA
GTGATCCTCATCCAAAGAGAAGTTTTAAAAGTTTAGCTCTTGATATTGAAGAAGTGGCTGATCAATTGGAACTTGGAAATAAATTTTATGTTATTGGATT
TGCAATGGGTGCACACTTTGTTTGGGGTTGCCTAAAGTACATACC

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.925881
Expected error %
0.1398
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g74280.1 Score 104 E Value 4e-23 Identity 21.10% Align. Length 227bp (35.19%) Frame 3
68414.m08602 hydrolase, alpha/beta fold family protein contains Pfam profile PF00561: hydrolase, alpha/beta fold family
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g36290.1 Score 101 E Value 3e-22 Identity 24.10% Align. Length 194bp (30.08%) Frame 3
68415.m04453 hydrolase, alpha/beta fold family protein low similarity to 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase [Rhodococcus sp. RHA1] GI:8978311; contains Pfam profile PF00561: hydrolase, alpha/beta fold family
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAO42354.1| Score 104 E Value 2e-21 Identity 21.10% Align. Length 227bp (35.19%) Frame 3
unknown protein [Arabidopsis thaliana] gb|AAO22607.1| unknown protein [Arabidopsis thaliana] ref|NP_565081.1| hydrolase, alpha/beta fold family protein [Arabidopsis thaliana] gb|AAG52399.1| unknown protein; 23197-21829 [Arabidopsis thaliana] pir||C96771 unknown protein F1O17.5 [imported] - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAM61430.1| Score 104 E Value 2e-21 Identity 21.10% Align. Length 227bp (35.19%) Frame 3
unknown [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0151N15
Clone
Clone name
hba-151n15
LE_HBa0151N15
P151N15
Clone type
BAC
Contamination
none
Estimated length
95,461
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found