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Clone Read LE_HBa0054D16_SP6_12078

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0054D16_SP6_12078
Primer
SP6
ID from Seq. Facility
LE_HBa_054_D16-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
582 trimmed for vector and quality, 897 raw
Genbank Accession
DU030874
Sequence
>LE_HBa0054D16_SP6_12078 (raw untrimmed)
CCCATAAATTTCTTTATCAGCGTTGTTGTCTGAGTTGCGACCACGGACGCCTTCTGGGCCAGGAATGTGTTGGACATGAAGCTTCTTGTCCTCAAAGCTG
AGGACCATCTCAGCCATCTCATTCATGCTGACCATCTCATCACTTCCAATGTTTACTGGCTCACGGAAGTCGGATTTTGTCAATCTATATAAAGAGTATT
AAATAAAACAAGCCACTTAGACAAAAGCTTATTCGACGACCATTGTTAAAAGTAATATTGAGCAACTAAAATTGATTTGTTTATATAAACTATAGCTAAG
TTTATTATATTTCTTCCAAAGTAACAAGTAAGGTCCCCCTTGAGGAATTCAATCTAATCGGCAAACACGTTCCCAAAAGAAAGAAATTCAAGGTGCCTAA
TTCTCATAACTGAATCCAATCAAAGCACAAAACACGACTTCTCCAGTAATTCAGAATAAGTGGATATTTGTAGTAACTAATTGCTTAATTGTGTACAGAA
GAAATCAAGATTTGGCTGTCTCACCTAAGAACCCCTTCAACACACTCATCAATGAAGGTGAATGAACGTGTTTGAAGTCCATCTCCCCACATTTTAAACT
TATCCGTTGCAGTTTGTGCTTTTCTACAAAAAGCGGCATGAGCTTTTTTCCCTTCCACCTGAAATTCAGAAAACATTAAGAAATATGACTTCTCACAATC
CATATAAAAAAAGAATGTAATTTGTCTGTATGCAACCACTTTCACTTAATTACCTCTTTCAGTTCCAAAAGGTCTTTGATGTTATGGAACCTTCCATACG
TCATCCAATTCCAATATCGTGGTGTGATGATTGTCAATTCTTTGTGGTGACGCCTCCCCAACCTTGGCTTCTTCAGCCTCACCCTCACCTCCAATTA
>LE_HBa0054D16_SP6_12078 (trimmed for quality and cloning vector)
GGACGCCTTCTGGGCCAGGAATGTGTTGGACATGAAGCTTCTTGTCCTCAAAGCTGAGGACCATCTCAGCCATCTCATTCATGCTGACCATCTCATCACT
TCCAATGTTTACTGGCTCACGGAAGTCGGATTTTGTCAATCTATATAAAGAGTATTAAATAAAACAAGCCACTTAGACAAAAGCTTATTCGACGACCATT
GTTAAAAGTAATATTGAGCAACTAAAATTGATTTGTTTATATAAACTATAGCTAAGTTTATTATATTTCTTCCAAAGTAACAAGTAAGGTCCCCCTTGAG
GAATTCAATCTAATCGGCAAACACGTTCCCAAAAGAAAGAAATTCAAGGTGCCTAATTCTCATAACTGAATCCAATCAAAGCACAAAACACGACTTCTCC
AGTAATTCAGAATAAGTGGATATTTGTAGTAACTAATTGCTTAATTGTGTACAGAAGAAATCAAGATTTGGCTGTCTCACCTAAGAACCCCTTCAACACA
CTCATCAATGAAGGTGAATGAACGTGTTTGAAGTCCATCTCCCCACATTTTAAACTTATCCGTTGCAGTTTGTGCTTTTCTA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.961771
Expected error %
0.457188
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g28840.1 Score 90 E Value 8e-19 Identity 91.50% Align. Length 579bp (99.48%) Frame 3
68418.m03547 NAD-dependent epimerase/dehydratase family protein similar to sugar epimerase BlmG from Streptomyces verticillus GI:9937230; contains Pfam profile PF01370 NAD dependent epimerase/dehydratase family
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAM51587.1| Score 90 E Value 3e-17 Identity 91.50% Align. Length 579bp (99.48%) Frame 3
AT5g28840/F7P1_20 [Arabidopsis thaliana] ref|NP_198236.1| NAD-dependent epimerase/dehydratase family protein [Arabidopsis thaliana] gb|AAL15324.1| AT5g28840/F7P1_20 [Arabidopsis thaliana] gb|AAL15291.1| AT5g28840/F7P1_20 [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAP53779.1| Score 87 E Value 3e-16 Identity 85.10% Align. Length 579bp (99.48%) Frame 3
putative epimerase/dehydratase [Oryza sativa (japonica cultivar-group)] ref|NP_921492.1| putative epimerase/dehydratase [Oryza sativa (japonica cultivar-group)] gb|AAM08784.1| Putative epimerase/dehydratase [Oryza sativa] dbj|BAD66930.1| GDP-mannose-3'',5''-epimerase [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0054D16
Clone
Clone name
hba-54d16
LE_HBa0054D16
P054D16
Clone type
BAC
Contamination
none
Estimated length
77,492
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found