Clone Read LE_HBa0189L05_SP6_106904

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0189L05_SP6_106904
Primer
SP6
ID from Seq. Facility
LE_HBa_189_L05-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
483 trimmed for vector and quality, 965 raw
Genbank Accession
DU092549
Sequence
>LE_HBa0189L05_SP6_106904 (raw untrimmed)
TCCGGGCTTTATTCCCCAGGAACTAAGTTGAATACTGCAAAAACCCGACGTTGCGGACAGGATAATAATATCCGATACTATGGATGAAGTTGATCATAAG
TGGCATGAACAGGAACAAAAATACGACTTTGAAAGTCATACAGATCAATGTGCTTTTAGAGTTAGCATATACAGAGGTCATCCTGAGTTCAGGAGAAGAA
GTCAGTGCAATGAACCGGGAAGGTTAATAAAGCTTCCCGATTCACTTGAAAAACTCAAGGGCATTGCAGGTTCCTTTTCAAACAAATTCTTCTCCATTTT
TAGTTCTTCCACAATCCTAACATTATCTATTTTGTAGGCCACAAGTTTGGCTTTGATGCAAAAGATGCATTGGTGACAAATGAAGAAGGAGCGGAAATTG
ACTCGGTTGAAGTCATTAGGGATAACGATAAGCTTTATATATATAGTTGAATATACAAACTCCATAATGTAGCATGAGCTGTTCTACTAGTATTTAAGTC
TTTGTTCTATTATTTACACTGCTATAAAATTTAAGAAAAGGATATTACCCTCTTTCACTCCTCCATTTGCTTACCTATCACGAGAACTCTTCCAATCTAT
ATCGACCGATTGACTCTTGTTCAACGAGAAAAAGCTGATGATTGGCACTGGTCCTATCATGTTTACTGTCGCGTTTTTACGGATTCTCTTTTGGTATCAA
GACCTCTAAATACCTCCTGTCCCGAATTAGTGATAAATTCTTGCTTTAGATGAACTCCTATCATGTACTCCATCTGCACCTCCCTTTATTCAAATAGATT
CGGTTTGCACATCCTCATCTTTGTCCTCTTATTCAAAGACTCTCACACCTGGCTCTCCCCCCCCAATCCTAATCTTCTCCTTACCTATCTCTACTCCTCT
TCCTCCTGTGTCCGCCTGCTCCGCCTCGCTCTACTCACTTAATTGCATAACCAAAACTATTCGTC
>LE_HBa0189L05_SP6_106904 (trimmed for quality and cloning vector)
AGGATAATAATATCCGATACTATGGATGAAGTTGATCATAAGTGGCATGAACAGGAACAAAAATACGACTTTGAAAGTCATACAGATCAATGTGCTTTTA
GAGTTAGCATATACAGAGGTCATCCTGAGTTCAGGAGAAGAAGTCAGTGCAATGAACCGGGAAGGTTAATAAAGCTTCCCGATTCACTTGAAAAACTCAA
GGGCATTGCAGGTTCCTTTTCAAACAAATTCTTCTCCATTTTTAGTTCTTCCACAATCCTAACATTATCTATTTTGTAGGCCACAAGTTTGGCTTTGATG
CAAAAGATGCATTGGTGACAAATGAAGAAGGAGCGGAAATTGACTCGGTTGAAGTCATTAGGGATAACGATAAGCTTTATATATATAGTTGAATATACAA
ACTCCATAATGTAGCATGAGCTGTTCTACTAGTATTTAAGTCTTTGTTCTATTATTTACACTGCTATAAAATTTAAGAAAAGG

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.953629
Expected error %
0.0729508
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g22200.1 Score 60 E Value 7e-20 Identity 68.40% Align. Length 280bp (57.97%) Frame 2
68417.m03209 potassium channel protein 2 (AKT2) (AKT3) identical to potassium channel [Arabidopsis thaliana] gi|1100898|gb|AAA97865; Note: also identical to AKT3 [Arabidopsis thaliana] gi|1172218|gb|AAA96153, which is a truncated version of AKT2, PMID:10852932; member of the 1 pore, 6 transmembrane (1P/6TM- Shaker-type) K+ channel family, PMID:11500563; identical to cDNA inward-rectifying K+ channel (AKT3) GI:1172219
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA70870.1| Score 140 E Value 5e-47 Identity 94.40% Align. Length 382bp (79.09%) Frame 2
putative inward rectifying potassium channel [Solanum tuberosum] pir||T07052 probable potassium channel protein SKT2 - potato
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAD81033.1| Score 97 E Value 6e-31 Identity 63.40% Align. Length 382bp (79.09%) Frame 2
potassium channel NKT2 [Nicotiana tabacum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0189L05
Clone
Clone name
hba-189l5
LE_HBa0189L05
P189L05
Clone type
BAC
Contamination
none
Estimated length
126,103
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found