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Clone Read LE_HBa0182M15_SP6_106204

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0182M15_SP6_106204
Primer
SP6
ID from Seq. Facility
LE_HBa_182_M15-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
723 trimmed for vector and quality, 801 raw
Sequence
>LE_HBa0182M15_SP6_106204 (raw untrimmed)
ATTGAAGTCCTTTCTTATTGAATTTCATGAGTTTCTTATTTGCATGCGAACAAGTAGTTCCTCTCTTTTCACTTAGAAACAGGAAAAATTTCATCCTATT
CACTGAGAGAGAACTATGTTACTTAATTTGCTGAATTTACTTTAAAAATAGTTAATTTAATTGATAGACCTTAGGACTTCATGTCAATCAGTCATTTTTT
TGTACATTTTACCACGTACCTTATTTGTGTGTATTGCATTTAAAAGCACAACTTTTAGAAGATACAGTTACCTCTGTATTACTAATGCAGGTATGCGACA
TATGGTACGGATTACGTTTACCCTCAGGTTAGTAGCACTGCATAAATGGAGACATTACAATACATCCCATTCTTTTTGCTGCACTTAAAGGTTCAGGTGT
CCTTTTGTCTTGACTATGAGGACTCATTTTTGATGCATACTGTAATTTTATCCGATAGGGTGTTTACAATCCTTATGGAGGTCAACAGTACCTTCCTATA
TATGGTGTACCTGGAACTGTTAATGCAACAATGTATCCTTACGGCCAGCTAAGTCAGGCTGTTCCTGGAAGTCACAGTTATACGACATTGCAGGGTTATG
CAGTGCCAGGACATCAGATTCTTCAGTTTGGTGGGCCCGGTGTCAATGCAATGACAGCTTCATCCATTCCAACTATTCAAGCACCATATCAAACAGGCAG
ATTTCTTTTATTTGTATCTTATATATGCATGCATGGTAGCCTTGCATATCATCTTATCATTGACATTAATCTGCATTTCCAGGTTATCAAGAAGTCTGAC
A
>LE_HBa0182M15_SP6_106204 (trimmed for quality and cloning vector)
ATTTCATGAGTTTCTTATTTGCATGCGAACAAGTAGTTCCTCTCTTTTCACTTAGAAACAGGAAAAATTTCATCCTATTCACTGAGAGAGAACTATGTTA
CTTAATTTGCTGAATTTACTTTAAAAATAGTTAATTTAATTGATAGACCTTAGGACTTCATGTCAATCAGTCATTTTTTTGTACATTTTACCACGTACCT
TATTTGTGTGTATTGCATTTAAAAGCACAACTTTTAGAAGATACAGTTACCTCTGTATTACTAATGCAGGTATGCGACATATGGTACGGATTACGTTTAC
CCTCAGGTTAGTAGCACTGCATAAATGGAGACATTACAATACATCCCATTCTTTTTGCTGCACTTAAAGGTTCAGGTGTCCTTTTGTCTTGACTATGAGG
ACTCATTTTTGATGCATACTGTAATTTTATCCGATAGGGTGTTTACAATCCTTATGGAGGTCAACAGTACCTTCCTATATATGGTGTACCTGGAACTGTT
AATGCAACAATGTATCCTTACGGCCAGCTAAGTCAGGCTGTTCCTGGAAGTCACAGTTATACGACATTGCAGGGTTATGCAGTGCCAGGACATCAGATTC
TTCAGTTTGGTGGGCCCGGTGTCAATGCAATGACAGCTTCATCCATTCCAACTATTCAAGCACCATATCAAACAGGCAGATTTCTTTTATTTGTATCTTA
TATATGCATGCATGGTAGCCTTG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.961475
Expected error %
0.230044
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g76460.1 Score 116 E Value 1e-26 Identity 20.80% Align. Length 239bp (33.06%) Frame 1
68414.m08893 RNA recognition motif (RRM)-containing protein low similarity to RRM-containing protein SEB-4 [Xenopus laevis] GI:8895698; contains Pfam profile: PF00076 RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g20880.1 Score 86 E Value 2e-17 Identity 17.10% Align. Length 227bp (31.40%) Frame 1
68414.m02615 RNA recognition motif (RRM)-containing protein similar to RRM-containing protein SEB-4 [Xenopus laevis] GI:8895698; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM); is the location of EST 197B1T7 , gb|AA597386
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAG51948.1| Score 116 E Value 5e-25 Identity 20.80% Align. Length 239bp (33.06%) Frame 1
putative RNA-binding protein; 24808-23340 [Arabidopsis thaliana] pir||D96792 probable RNA-binding protein, 24808-23340 [imported] - Arabidopsis thaliana gb|AAF16655.1| putative RNA-binding protein; 16955-18423 [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAN18091.1| Score 116 E Value 5e-25 Identity 20.80% Align. Length 239bp (33.06%) Frame 1
At1g76460/F15M4.4 [Arabidopsis thaliana] gb|AAK52999.1| F15M4.4/F15M4.4 [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0182M15
Clone
Clone name
hba-182m15
LE_HBa0182M15
P182M15
Clone type
BAC
Contamination
none
Estimated length
83,621
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found