Clone Read LE_HBa0172B01_SP6_104112

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0172B01_SP6_104112
Primer
SP6
ID from Seq. Facility
LE_HBa_172_B01-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
492 trimmed for vector and quality, 929 raw
Genbank Accession
DU092697
Sequence
>LE_HBa0172B01_SP6_104112 (raw untrimmed)
CCCACGATATCGTCAATAGTATCATCTAAGTACTCATCCTCTGAATTATGGTCAATCTTTTCAAGTGATTCACTCTTTAAAAGCCACCCATCAATCGTCA
TTGTCTTCACCATTCCCTTAAAGGACGATTTCACTTTTAACCATTGAACTAGACGCTTTTTTTTGTAATGATTCGCATAGTTCTAATAATTCTTGTTTTG
TCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGATTGTTATACCCTTCAGCTGGATA
CAACTCCCCTACTTTGTACTTGTGTTGATTGTGCTCTATGTCTTTAAAAGCTCTAACAACTTTAAATTTCACCATTTTATCACCTCATAAAATTTTATAG
TGTTTCTTCGGTACCTTCTAAAGCTGGCTTATGTCCTTTTAAATCTAATTTCCCAACAGCAGCAACTTTATTATCTTTCGCTTTGCCGTAAGCAAATTGT
TTTGCAGTGTATAAATCCATATCATCTAACGCAAGTGTTTCTTTAAATTTCTGAACATTAATACCACCAGCTAAATAACCATCATATAAACCTTTAACGT
ACGTTAAAACCTTACCTGCTTCTTGGACTGTAGACTCGATAACATTCAAATTAAATGGTAAAGCAGTAACATATACGCCATTTGCATTTAAATGTGTATA
CTGTGCTTGAACCCCAAAAGCATCGGACGGATTAACAACCATTGTTAACTTACCTTTAACCGCTACTGATTTACCTTTCTCGTTAGTTGAGTGGGATTAA
AACACCTGCCGCAATCCTTAAATGTAACGCGCGGATTAAAAAAAGAAAGCGAACCTTGGTCTTCTTCTCCGGGAAAACCCCCCCAGTTACCGAATCCCCT
TTTTGACTTGAAGGTTTAAGCCAATGGTT
>LE_HBa0172B01_SP6_104112 (trimmed for quality and cloning vector)
TTTCACTTTTAACCATTGAACTAGACGCTTTTTTTTGTAATGATTCGCATAGTTCTAATAATTCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAAC
TTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGATTGTTATACCCTTCAGCTGGATACAACTCCCCTACTTTGTACTTGTGTTGA
TTGTGCTCTATGTCTTTAAAAGCTCTAACAACTTTAAATTTCACCATTTTATCACCTCATAAAATTTTATAGTGTTTCTTCGGTACCTTCTAAAGCTGGC
TTATGTCCTTTTAAATCTAATTTCCCAACAGCAGCAACTTTATTATCTTTCGCTTTGCCGTAAGCAAATTGTTTTGCAGTGTATAAATCCATATCATCTA
ACGCAAGTGTTTCTTTAAATTTCTGAACATTAATACCACCAGCTAAATAACCATCATATAAACCTTTAACGTACGTTAAAACCTTACCTGCT

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.928288
Expected error %
0.489085
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|YP_041429.1| Score 166 E Value 2e-40 Identity 33.30% Align. Length 245bp (49.80%) Frame 3
hypothetical protein SAR2060 [Staphylococcus aureus subsp. aureus MRSA252] ref|NP_835557.1| hypothetical protein SA1773 [Staphylococcus phage phiN315] emb|CAG41044.1| hypothetical phage protein [Staphylococcus aureus subsp. aureus MRSA252] dbj|BAB58124.1| hypothetical protein [Staphylococcus aureus subsp. aureus Mu50] ref|NP_375071.1| hypothetical protein SA1773 [Staphylococcus aureus subsp. aureus N315] dbj|BAB43050.1| hypothetical protein [Staphylococcus aureus subsp. aureus N315] pir||A89986 hypothetical protein SA1773 [imported] - Staphylococcus aureus (strain N315) ref|NP_372486.1| hypothetical protein SAV1962 [Staphylococcus aureus subsp. aureus Mu50]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_958607.1| Score 164 E Value 9e-40 Identity 32.90% Align. Length 245bp (49.80%) Frame 3
77ORF045 [Bacteriophage 77] gb|AAR87913.1| 77ORF045 [Bacteriophage 77]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0172B01
Clone
Clone name
hba-172b1
LE_HBa0172B01
P172B01
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found