Clone Read LE_HBa0162E12_T7_103511

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0162E12_T7_103511
Primer
T7
ID from Seq. Facility
LE_HBa_162_E12-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
2 trimmed for vector and quality, 1158 raw
Sequence
>LE_HBa0162E12_T7_103511 (raw untrimmed)
CAGCGTACGTACCTGGCAGCGACGCGTGTGGATACCTCTCTGGAGTACATACTTAGCCTAGTGCAGGTTCAAGCCTTTTAAAACTATAGCATGGACCCGC
ACTCGAACCCCCGTCTCTGTTGATCACCGAGCGCACACACACATTGNCCAGGCTACATGGTGAGCGCATCCGCTGTCGTACGTGTGGAGGNGTGTACATT
TGAGTAATATTATCTCCTAGCATCTTCCCCCGCGTCGGCTTGTATGGGTTAACGATTGAGACGAAAATGCATTGTCATCGTCTAATCGAGTGCTTNAACA
GATTTCGATACCATCAGCTAGGACATGATAATTCTATGATTTGCCTGCCACTTCCCTGCACCTATATGACCCCGTGTCATGCTCCGTGGTCAGCGCGGGA
CGGCTGCCGCGGGACCACCCCGCGCCGAGCACCAGACCAAGATGTTTTACGAACAGCGTGTCGGCATGCGCGTACCCTACTTTTATATTGTCATAGTCTC
TTTCTTTGTGTGCTCACTGACTATAGCAAGTCCTAATATAAATATTGAGTCACGGGAGTTGAAATCTACTATGGTTGTACTTTGGTGACGACATATCTGT
TCGCAATCCTGAGTGCAGACCCGATAACGCCACAGCGAGAAATCGAAAGTCACAGTCAAGAAGGTCGGTGAGGACATCGAGAAAAGCATCCCTCAGCAAG
CCTCTCGATTCTAAATGCTGGCTCTCGAGGACACGTCTACGCCGCAGACCTCTCACTGAACCCTACGGGTGACCCTACTAAATCCTCTAAGCTTACATGT
ATCCCATCATCTATCATGTCCACATGCTGAAGAGAGCGATAATCAGAAGAGAACAGATTAAACTAGACACGGCGTCGGTCGAGTAAGAAGAACAGCTAAA
GGAACGAAGACTACGCAAGTGTGTGAATGAGATGGTGCGTGAAGCTTGCTTCTTCCTCACTTTGCTTAAGTGTGCTCTGTACTGAAGCGTTGTGCGCTGG
CGTGATGGAGCAGGCGTCGCGGAAGCTCACTGTAGACGCCCATACCGCCGGGGCTGTCTCATGTGTCGGATGTCGCCAACTGCCGCGTCAAATCCAGAAA
CAGCAAGAGCCTGTCACAGTACCATTCAAATGGGCGCAATACAGACAACTCTCTACAC
>LE_HBa0162E12_T7_103511 (trimmed for quality and cloning vector)
CT

This is probably not a useful sequence.

Problems:
  • Short cloning insert
  • Low complexity
  • High predicted error rate
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
not recorded
Expected error %
4.36881
Sequence Annotations 
Automatic Annotations
No automatic annotations found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0162E12
Clone
Clone name
hba-162e12
LE_HBa0162E12
P162E12
Clone type
BAC
Contamination
none
Estimated length
112,064
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found