Clone Read LE_HBa0158N21_T7_103193

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0158N21_T7_103193
Primer
T7
ID from Seq. Facility
LE_HBa_158_N21-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
239 trimmed for vector and quality, 924 raw
Sequence
>LE_HBa0158N21_T7_103193 (raw untrimmed)
TTGTTCCCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTTCTCCCTTAACATTTCCAATACCCCTCTCAAGGGCTCTTCATGCTCCTTCT
TGCTCGTTGAGTACCCCTCTATATCATCACTAAATACGATCACTAAGAGGTCCCAATATTGGCTTAAAAATGCTGTTCATCATGCTCATTAACCCCGCCC
GGGCATTCGTTAGACCAAAAGACTTCACTACAAATTCGTAATGCCCATACCTCGATCCAAAAGCAGTCTTTTGGCACATCCCGGTTGTCTCGTATTTCCC
TATTGATTCATAACCGGCATCTCCAGAACAAATCTTTATGGAATAGATATAAGCTCTCTCGTCACTTAGGCGTATCTAGTGTTTTTTTTTTCGGGTTAAT
ATAAGCCCGAGAGTTGCCGCTGCACGTCGAGTATCGATACTCATCATGTCGTGCTCACTTTATTGTAGATAACTACTTTTATGATATCTCTATATTAATC
AACAATGTTTGTCTACAGATTATGACAACACATCATCTCCCTCTTTCTTTCTTGGCTTTCTCTGTCTTATCCTAGGTATTAATCATTCGATTTTATTTTA
AAGATAATCTCGTAACCATATTTATTTTGAAGTGTGATTTTGTGGATAAGTGGCTAGATTATTTTTTGTTGCTTTGTGACGAATCTTTGCTGCTTTCCTA
TCTTAGTATATATATTTCTTTTACTTTGCGTCCTTGCATCGCTTGTCCACCTCGTGTGCAGTAATGACTGCATCTTTTAACGTTCCGAAGTTATGTATGT
CTTGGCCCGCATATGGAGCAATCATCCTCGTTGCGCCCACATAACAATGTGCAGTCGTTTGCCCATCTTGCGCTCTCGGTGCGCTTTGTGGTCTGTTTTG
CTAGATACGCCGAACTGTCCGACG
>LE_HBa0158N21_T7_103193 (trimmed for quality and cloning vector)
AGCTTTTTCTCCCTTAACATTTCCAATACCCCTCTCAAGGGCTCTTCATGCTCCTTCTTGCTCGTTGAGTACCCCTCTATATCATCACTAAATACGATCA
CTAAGAGGTCCCAATATTGGCTTAAAAATGCTGTTCATCATGCTCATTAACCCCGCCCGGGCATTCGTTAGACCAAAAGACTTCACTACAAATTCGTAAT
GCCCATACCTCGATCCAAAAGCAGTCTTTTGGCACATCC

This is probably not a useful sequence.

Problems:
  • High predicted error rate
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.951793
Expected error %
4.38776
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT85839.1| Score 59 E Value 2e-14 Identity 70.00% Align. Length 228bp (95.40%) Frame 1
reverse transcriptase [Vigna radiata]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT85838.1| Score 59 E Value 2e-14 Identity 70.00% Align. Length 228bp (95.40%) Frame 1
reverse transcriptase [Vigna radiata]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0158N21
Clone
Clone name
hba-158n21
LE_HBa0158N21
P158N21
Clone type
BAC
Contamination
none
Estimated length
111,245
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found