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Clone Read LE_HBa0158E08_SP6_102734

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0158E08_SP6_102734
Primer
SP6
ID from Seq. Facility
LE_HBa_158_E08-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
716 trimmed for vector and quality, 934 raw
Genbank Accession
DU093309
Sequence
>LE_HBa0158E08_SP6_102734 (raw untrimmed)
CCCGGGCTTTTGTCAATCACAATTAGTATCAGCAGCAATATCTCTGCATTACATGGACTTGAGATATAGCTGAACTTTATAAGGCACGCTTTCTTTTCAT
GAGATCGATAAACAACCTTACTTCAATACAATTAAGCATGTTGAAAACTTTAAGAATGAATTGTTTGAGGAAGTGTTAATTTGGTATCACTAATTCTATT
CTCCAGGGTAAGGAAGTGATGTAATAATTCGGTACATTATCAAAATATGCAGAGGCACACTACATGTTCAGCAAAATAAAAAATTGGACGATCGGAAGTG
CTGCAACAAAAATAAACTTACTTTGATTGCTGAGAACATCGGAGGGACTTGCCATATCTCTCCAAAAAAGGATGCTGCCGTTTTCTTTATGTCCTCATCC
TTGATATGCTCCCATGGCTCTCGTTGAATAACCTGAACATTGCAAAATCCAAAATTCATAGGTAAGATGAAACCTGGGGTAAACAAATAACTAGATCCTG
TTTATGTAACATCAACTAGTGTGAGTGTGTAGACTTTATTCATGATGGCTTTATCACGAAATTTCTCATCTTTCCATCTTAATTTGTGTTGCTGATGATT
AAATGAGACTTTCGTGTGGGTGAAATAAGCCACACATTCCAGATTTATCTGAAGAAATATAACTTCAAAAAAAACAACTAAATACCGGGGAATCTGCATC
CCAGGTAGAGGTGGCTTCTCCCAAACGAAGATCCCACTGTAGCCCTTAATCATACCCTGATAGCTGCCAAGAACAATTCAACTTAAGTTATTACATAACT
TCCCATAAACAGGAATAGTTCTCTTAAAAAAGAGGAAATTAGCAGGAAGAAAGCCTCATGAAATTTAAGGAACCCTGAAATCTCCCCCCATTTAATCTCT
AAAACCTTATTACCTGTCACAATCTTTGTAAATT
>LE_HBa0158E08_SP6_102734 (trimmed for quality and cloning vector)
AGTATCAGCAGCAATATCTCTGCATTACATGGACTTGAGATATAGCTGAACTTTATAAGGCACGCTTTCTTTTCATGAGATCGATAAACAACCTTACTTC
AATACAATTAAGCATGTTGAAAACTTTAAGAATGAATTGTTTGAGGAAGTGTTAATTTGGTATCACTAATTCTATTCTCCAGGGTAAGGAAGTGATGTAA
TAATTCGGTACATTATCAAAATATGCAGAGGCACACTACATGTTCAGCAAAATAAAAAATTGGACGATCGGAAGTGCTGCAACAAAAATAAACTTACTTT
GATTGCTGAGAACATCGGAGGGACTTGCCATATCTCTCCAAAAAAGGATGCTGCCGTTTTCTTTATGTCCTCATCCTTGATATGCTCCCATGGCTCTCGT
TGAATAACCTGAACATTGCAAAATCCAAAATTCATAGGTAAGATGAAACCTGGGGTAAACAAATAACTAGATCCTGTTTATGTAACATCAACTAGTGTGA
GTGTGTAGACTTTATTCATGATGGCTTTATCACGAAATTTCTCATCTTTCCATCTTAATTTGTGTTGCTGATGATTAAATGAGACTTTCGTGTGGGTGAA
ATAAGCCACACATTCCAGATTTATCTGAAGAAATATAACTTCAAAAAAAACAACTAAATACCGGGGAATCTGCATCCCAGGTAGAGGTGGCTTCTCCCAA
ACGAAGATCCCACTGT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.966729
Expected error %
0.588657
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g14460.1 Score 71 E Value 5e-13 Identity 28.10% Align. Length 113bp (15.78%) Frame 2
68418.m01692 pseudouridylate synthase TruB family protein similar to SP|P09171 tRNA pseudouridine synthase B (EC 4.2.1.70) (tRNA pseudouridine 55 synthase) (Pseudouridylate synthase) (Uracil hydrolyase) {Escherichia coli O157:H7}; contains Pfam profile PF01509: TruB family pseudouridylate synthase (N terminal domain)
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAO33771.1| Score 77 E Value 6e-13 Identity 30.70% Align. Length 113bp (15.78%) Frame 2
putative tRNA pseudouridine synthase [Oryza sativa (indica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAU44133.1| Score 77 E Value 6e-13 Identity 30.70% Align. Length 113bp (15.78%) Frame 2
putative tRNA synthase [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0158E08
Clone
Clone name
hba-158e8
LE_HBa0158E08
C02HBa0158E08
P158E08
Clone type
BAC
Contamination
none
Estimated length
114,102
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found