Clone Read LE_HBa0144F17_T7_100497

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0144F17_T7_100497
Primer
T7
ID from Seq. Facility
LE_HBa_144_F17-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
833 trimmed for vector and quality, 908 raw
Genbank Accession
DU091366
Sequence
>LE_HBa0144F17_T7_100497 (raw untrimmed)
CAGAACTTGGCCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAAAGTTGAGGCTTTCAAGATTATTGGACAGGCATCTACAACCTTATCA
GATGAACATCGCAAAGAGATTCCTTAATGCACAAATTACCACAAAACTAAGCTAAAAAGTTGAGGCTTTTGAGATTATTGGACAGGCATCTACAACCTTA
TCATGTGAACATCAGTAATGGTATACGATAACGAGATATGAAGCTAAAGAACAACTAAAGTGCCTATTAACCAAACAAAAACATAAATCACAGAGCAAGA
AGAAGGTGGTTTACCTGATCAACGTATTGACTTTCTTTGCCTGGATGTCATACATTTTCTTCACCGCATCCCTGATCTTCTTTTTGTCAGCATGAATGTC
AACAATAAATACAAGTGTATTATTATCCTCAATTTTCTTCATTGCAGACTCGGTGGTTAGAGGACATTGAAGAATCTGGTACTGATCAAGTCTGTTCCTT
CCAGGTGCACTAATCCTGGGATACTTGGGATTTCTGTCCTTCTTCAATGTCTTAGGCCGATGAAATGTAACTTTTGTTCGTATTCTTTTTGACATCTTCT
TAAAGGTTGATCCCGACTTCACAGCCTTGGCAGCCTTTACAGCTTGAGCTTTGGGGTCACCTTTTTTTATCACATCTGCTGCATTGCCAATTCCAAGTCC
AGACTAAGTAAAACAGATCAGGTATCTCTACTAGCACGAATATATGCAACAGAAATTCATTGATCAAATTTTCTCATGCAAATCACTTTTTGATACTTCA
ACTCGCATCACTTTCTGTTTCTGTTGCATCACGAAATTCCAATAGATAAACAGTACTAGTAAACCATATAAATCTTACACCTAATACCAGTCTACCTAAA
AAGGCCCC
>LE_HBa0144F17_T7_100497 (trimmed for quality and cloning vector)
AGCTTAAAGTTGAGGCTTTCAAGATTATTGGACAGGCATCTACAACCTTATCAGATGAACATCGCAAAGAGATTCCTTAATGCACAAATTACCACAAAAC
TAAGCTAAAAAGTTGAGGCTTTTGAGATTATTGGACAGGCATCTACAACCTTATCATGTGAACATCAGTAATGGTATACGATAACGAGATATGAAGCTAA
AGAACAACTAAAGTGCCTATTAACCAAACAAAAACATAAATCACAGAGCAAGAAGAAGGTGGTTTACCTGATCAACGTATTGACTTTCTTTGCCTGGATG
TCATACATTTTCTTCACCGCATCCCTGATCTTCTTTTTGTCAGCATGAATGTCAACAATAAATACAAGTGTATTATTATCCTCAATTTTCTTCATTGCAG
ACTCGGTGGTTAGAGGACATTGAAGAATCTGGTACTGATCAAGTCTGTTCCTTCCAGGTGCACTAATCCTGGGATACTTGGGATTTCTGTCCTTCTTCAA
TGTCTTAGGCCGATGAAATGTAACTTTTGTTCGTATTCTTTTTGACATCTTCTTAAAGGTTGATCCCGACTTCACAGCCTTGGCAGCCTTTACAGCTTGA
GCTTTGGGGTCACCTTTTTTTATCACATCTGCTGCATTGCCAATTCCAAGTCCAGACTAAGTAAAACAGATCAGGTATCTCTACTAGCACGAATATATGC
AACAGAAATTCATTGATCAAATTTTCTCATGCAAATCACTTTTTGATACTTCAACTCGCATCACTTTCTGTTTCTGTTGCATCACGAAATTCCAATAGAT
AAACAGTACTAGTAAACCATATAAATCTTACAC

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.963564
Expected error %
0.534735
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g39460.1 Score 171 E Value 6e-43 Identity 24.20% Align. Length 362bp (43.46%) Frame 1
68415.m04843 60S ribosomal protein L23A (RPL23aA) identical to GB:AF034694
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At3g55280.1 Score 170 E Value 1e-42 Identity 24.20% Align. Length 362bp (43.46%) Frame 1
68416.m06139 60S ribosomal protein L23A (RPL23aB) various ribosomal L23a proteins
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||S48026 Score 189 E Value 8e-47 Identity 26.50% Align. Length 365bp (43.82%) Frame 1
ribosomal protein L23a, cytosolic - common tobacco sp|Q07761|RL23A_TOBAC 60S ribosomal protein L23a (L25) gb|AAA53296.1| 60S ribosomal protein L25
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA63112.1| Score 174 E Value 3e-42 Identity 25.70% Align. Length 353bp (42.38%) Frame 1
ribosomal protein L23 [Spinacia oleracea]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0144F17
Clone
Clone name
hba-144f17
LE_HBa0144F17
P144F17
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found