Clone hba-326k10

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Clone
Clone name
hba-326k10
LE_HBa0326K10
C12HBa0326K10
P326K10
Clone type
BAC
Contamination
none
Estimated length
74,107
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg1718 : [coherent], 1 members:
C12HBa0326K10
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
TG468Tomato-EXPEN 2000plate 16A12
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 12
FISH map 
View on FISH map chromosome 12
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
678-02 1CompositeFluorescencePhase80.7%
678-05 1CompositeFluorescencePhase85.1%
678-07 1CompositeFluorescencePhase83.7%
678-22 1CompositeFluorescencePhase82.2%
678-26 1CompositeFluorescencePhase81.2%
678-30 1CompositeFluorescencePhase80.4%
678-32 1CompositeFluorescencePhase80.7%
678-33 1CompositeFluorescencePhase83.4%
678-43 1CompositeFluorescencePhase82.5%
678-46 1CompositeFluorescencePhase84.4%
Mean±sd82.4%±1.7%

Given an estimated length of 7.3µm for chromosome 12Q and the mean percentage above, this BAC is approximately 6.0µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 12 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 12
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
134,255 bp
C12HBa0326K10.1
AC209663.1 (GenBank) 		[View]
End Sequences
None
Warnings
  • poor correspondence with in vitro restriction fragments
    (match score below 0.7)
  • sequence length 134,255 is 81.2% different from estimated length 74,107
 FTP
Chromosome 12 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (20)
1987, 1987, 2060, 2079, 2268, 2440, 2440, 2544, 2867, 3195, 3246, 3515, 3536, 4048, 4704, 5650, 5650, 5850, 6478, 7563
HindIII in silico (46)
1027, 1053, 1081, 1114, 1138, 1159, 1178, 1178, 1204, 1209, 1266, 1415, 1450, 1567, 1578, 1730, 1839, 1911, 1968, 1983, 1990, 2005, 2085, 2272, 2274, 2431, 2457, 2478, 2554, 2567, 2776, 2864, 2897, 3214, 3302, 3551, 3629, 4091, 4575, 5282, 5357, 5428, 5614, 6427, 7295, 7566
Fragment Lengths Match Score
0.65
Annotations  
Browse
LE_HBa0326K10 has no browsable sequence annotations
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 and  bp
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End Sequences

e.g. crystallin
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Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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