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Clone hba-245e5

Clone and library  
Clone
Clone name
hba-245e5
LE_HBa0245E05
C05HBa0245E05
P245E05
Clone type
BAC
Contamination
none
Estimated length
94,363
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg5142 : [coherent], 2 members:
LE_HBa0140K15, C05HBa0245E05
Marker matches - overgo what's this? 
Plausible matches Additional Info
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
FISH map 
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
296-02b 1CompositeFluorescencePhase87.6%
296-04a 1CompositeFluorescencePhase85.3%
301-06 1CompositeFluorescencePhase89.0%
296-07 1CompositeFluorescencePhase86.3%
301-08 1CompositeFluorescencePhase86.1%
296-09b 1CompositeFluorescencePhase89.9%
296-10 1CompositeFluorescencePhase89.0%
301-10 1CompositeFluorescencePhase87.1%
296-11 1CompositeFluorescencePhase89.4%
301-11 1CompositeFluorescencePhase88.8%
296-17 1CompositeFluorescencePhase88.2%
296-21 1CompositeFluorescencePhase88.6%
301-22 1CompositeFluorescencePhase90.1%
296-24 1CompositeFluorescencePhase87.0%
296-25 1CompositeFluorescencePhase89.2%
296-28 1CompositeFluorescencePhase89.7%
Mean±sd88.2%±1.5%

Given an estimated length of 9.7µm for chromosome 9Q and the mean percentage above, this BAC is approximately 8.6µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 5 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 5
HTGS phase
2 - fragmented, ordered
Sequencing Status
in_progress
Sequencing Organization
Indian Agricultural Research Institute
Full Sequence
122,023 bp
C05HBa0245E05.1
AC186297.1 (GenBank)
[View]
End Sequences
Warnings
  • sequence is not finished to HTGS3
FTP
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (28)
1031, 1115, 1199, 1310, 1381, 1545, 1595, 1974, 2019, 2019, 2114, 2491, 2622, 2751, 3059, 3082, 3359, 3684, 3806, 3806, 4106, 5197, 5573, 5573, 5911, 6398, 6686, 8957
HindIII in silico (37)
1012, 1014, 1031, 1099, 1116, 1183, 1189, 1206, 1301, 1318, 1333, 1379, 1549, 1601, 1944, 2011, 2015, 2105, 2517, 2637, 2750, 3035, 3089, 3368, 3737, 3836, 3850, 3877, 4249, 4978, 5210, 5274, 5339, 5620, 6285, 6639, 8718
Fragment Lengths Match Score
0.87
Annotations  
Browse
LE_HBa0245E05 has no browsable sequence annotations
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