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Clone hba-140o23

Clone and library  
Clone
Clone name
hba-140o23
LE_HBa0140O23
C01HBa0140O23
P140O23
Clone type
BAC
Contamination
none
Estimated length
83,773
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg2236 : [coherent], 2 members:
C01HBa0140O23, LE_HBa0246F17
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
T1649Tomato-EXPEN 2000plate 9D5
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 1
FISH map 
View on FISH map chromosome 1
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
657-06 1CompositeFluorescencePhase59.0%
657-11 1CompositeFluorescencePhase58.9%
657-16 1CompositeFluorescencePhase57.6%
657-19 1CompositeFluorescencePhase59.4%
657-24 1CompositeFluorescencePhase61.2%
657-28 1CompositeFluorescencePhase55.8%
657-29 1CompositeFluorescencePhase62.0%
657-32 1CompositeFluorescencePhase59.8%
657-36 1CompositeFluorescencePhase58.2%
657-38 1CompositeFluorescencePhase59.8%
657-40 1CompositeFluorescencePhase60.0%
657-43 1CompositeFluorescencePhase57.1%
657-49 1CompositeFluorescencePhase58.9%
657-50 1CompositeFluorescencePhase56.6%
657-56 1CompositeFluorescencePhase58.9%
657-58 1CompositeFluorescencePhase56.8%
657-60 1CompositeFluorescencePhase56.1%
Mean±sd58.6%±1.7%

Given an estimated length of 20.1µm for chromosome 1Q and the mean percentage above, this BAC is approximately 11.8µm±0.4µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 1 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 1
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Uploaded by account 'manual'
Full Sequence
126,308 bp
C01HBa0140O23.1
AC238911.3 (GenBank) 		[View]
End Sequences
Warnings
  • sequence length 126,308 is 50.8% different from estimated length 83,773
 FTP
Chromosome 1 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (21)
1548, 1873, 2002, 2002, 2126, 2126, 2426, 2593, 2901, 2964, 3124, 3237, 3354, 3822, 3956, 4218, 5130, 6065, 6896, 7513, 13897
HindIII in silico (29)
1025, 1068, 1097, 1383, 1425, 1539, 1881, 1974, 2009, 2106, 2128, 2445, 2603, 2877, 2930, 3132, 3226, 3364, 3869, 4000, 4221, 4877, 5785, 6901, 7279, 7539, 7557, 13424, 13627
Fragment Lengths Match Score
0.83
Annotations  
Browse
LE_HBa0140O23 has no browsable sequence annotations
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 and  bp
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End Sequences

e.g. crystallin
Overgo match to marker what's this?
Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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