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Clone hba-298c3

Clone and library  
Clone
Clone name
hba-298c3
LE_HBa0298C03
C05HBa0298C03
P298C03
Clone type
BAC
Contamination
none
Estimated length
103,271
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
Tomato FPC (AGI 2005)
ctg2078 : [coherent], 4 members:
LE_HBa0044E04, LE_HBa0109E08, LE_HBa0190E11, C05HBa0298C03
Marker matches - overgo what's this? 
Plausible matches
ProbeMapPlateWell
TG96Tomato-EXPEN 2000plate 5H7
 Additional Info
Overgo Plating Progress Report
Conflicted matches
None
  
Marker matches - BLASTNone
Marker matches - manualNone
Physical map (from marker matches) 
View on physical map chromosome 5
FISH map 
View on FISH map chromosome 5
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
337-04 1CompositeFluorescencePhase53.9%
337-06 1CompositeFluorescencePhase54.8%
337-08 1CompositeFluorescencePhase55.7%
337-10 1CompositeFluorescencePhase53.4%
337-12 1CompositeFluorescencePhase57.6%
337-15 1CompositeFluorescencePhase52.9%
337-18 1CompositeFluorescencePhase56.0%
337-20 1CompositeFluorescencePhase54.0%
337-23 1CompositeFluorescencePhase53.5%
337-27 1CompositeFluorescencePhase52.8%
337-29 1CompositeFluorescencePhase57.2%
337-30 1CompositeFluorescencePhase58.9%
337-32 1CompositeFluorescencePhase53.4%
337-33 1CompositeFluorescencePhase59.2%
337-35 1CompositeFluorescencePhase53.8%
337-36a 1CompositeFluorescencePhase54.1%
337-36b 1CompositeFluorescencePhase55.0%
337-37a 1CompositeFluorescencePhase48.8%
337-37b 1CompositeFluorescencePhase53.9%
Mean±sd54.7%±2.4%

Given an estimated length of 7.4µm for chromosome 5P and the mean percentage above, this BAC is approximately 4.0µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 5 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 5
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
Indian Initiative on Tomato Genome Sequencing
Full Sequence
116,246 bp
C05HBa0298C03.2
AC212303.2 (GenBank) 		[View]
End Sequences
None
Warnings
none
FTP
Chromosome 5 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (23)
2029, 2043, 2248, 2248, 2366, 2435, 2771, 2771, 3274, 3653, 3798, 3844, 4010, 4102, 4288, 5035, 5895, 5895, 6634, 6634, 7145, 8010, 12143
HindIII in silico (28)
1009, 1196, 1703, 1787, 2018, 2036, 2258, 2263, 2292, 2421, 2474, 2782, 2851, 3316, 3715, 3862, 3907, 4067, 4149, 4258, 4312, 4839, 5602, 5627, 6637, 7184, 7944, 12062
Fragment Lengths Match Score
0.88
Annotations  
Browse
LE_HBa0298C03 has no browsable sequence annotations
Download
temporarily unavailable
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 More Criteria

Seq. Center Reported Status
Estimated Length
 and  bp
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e.g. AP009318.1 or AP009318
Sequencing on Chromosome
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Annotations
End Sequences

e.g. crystallin
Overgo match to marker what's this?
Map
Map position
 and  cM
Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
 Verification

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