Library Detail Page for LePU
Short Name: | LePU |
Organism: | Solanum lycopersicum (formerly Lycopersicon esculentum) |
Library Name: | Normalized cDNA library from ripening tomato pericarp |
Total Sequences: | 1495 sequences from 1542 clones |
Average Sequence Length: | 498.4 (Standard deviation 137.9) |
Type: | cDNA Library |
Tissue: | Pericarp |
Development Stage: | Turning stage of fruit ripening. |
Treatment Conditions: | |
Cloning Host: | |
Cloning Kit: | |
Comments | Equal amounts of total RNA isolated from two tomato genotypes differing in juice Bostwick value were mixed and used to prepare a normalized cDNA library (Takahashi-N and Ko-MSH, 1994: Toward a whole cDNA catalog: construction of an equalized cDNA library from mouse embryos. Genomics-San-Diego, 23: 1, 202-210). The resultant PCR amplified products were ligated into pBluscript SKII- vector (Stratagene) and transformed into E. coli DH5alpha |
Authors | Srivastava,A., Datsenka,T., Kudla,K., Bhaskaran,A. and Handa,A.K. |
Contact Information |