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Quiescent Seed Library

Library: cLEE
TIGR ID: TSE
Authors: Alcala, Vrebalov, White, and Giovannoni
Date made: 12/99
Species: Lycopersicon esculentum
Accession: TA496
Tissue: seeds
Developmental stage: dissected five days post-anthesis to fruit over-ripe stage
Vector: pBluescript SK(+/-)
Host: SOLR
Primary pfu: 1.2 x 104
Number mass excised: 1.2 x 104
Average insert length: 1.0 Kb
Cloning sites: 5' EcoRI, 3' XhoI
Antibiotic: ampicillin
Primers: M13F and M13R
Comments: All of the primary library was mass excised. Tissue is from seed and maturing carpels. Fruit from 5 dpa, 10 dpa, 20 dpa, mature green, breaker, firm, red-ripe, and very soft, red-ripe were collected. Seed was removed from all stages, though there was relatively little from the 5 dpa stage. 0.1 g of 5 dpa seed was combined with 0.3 g of 10 dpa, 0.7 g of 20 dpa, and 0.3 g from each of the mature green through late ripening stage seeds (1.2 g total for these 4) for RNA extraction.