_Lycopersicon pennellii_ Trichome Library

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Lycopersicon pennellii Trichome Library

Library:	cLPT
TIGR ID:	TPT
Authors:	Rutger van der Hoeven and Steve Tanksley
Date made:	06/99
Species:	Lycopersicon pennellii
Accession:	LA716
Tissue:	general collection of all trichome types
Developmental stage:	trichomes from leaves of 4-8 week old plants
Vector:	pBluescript SK(+/-)
Host:	SOLR
Primary pfu:	2.5 x 10⁷
Number mass excised:	1.5 x 10⁶
Average insert length:	1.2 Kb
Cloning sites:	5' EcoRI, 3' XhoI
Antibiotic:	ampicillin
Primers:	M13F and M13R
Comments:	Leaves of various stages were shaken in liquid nitrogen, shearing off trichomes. this procedure yielded a mixture of cells which is highly enriched in trichome. Note that it likely will contain minor contaminations of other types of leaf cells.

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