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Lycopersicon hirsutum Trichome Library

Library: cLHT
TIGR ID: THT
Authors: Rutger van der Hoeven and Steve Tanksley
Date made: 06/99
Species: Lycopersicon hirsutum
Accession: LA1777
Tissue: general collection of all trichome types
Developmental stage: trichomes from leaves of 4-8 week old plants
Vector: pBluescript SK(+/-)
Host: SOLR
Primary pfu: 3.0 x 106
Number mass excised: 1.5 x 106
Average insert length: 1.8 Kb
Cloning sites: 5' EcoRI, 3' XhoI
Antibiotic: ampicillin
Primers: M13F and M13R
Comments: Leaves of various stages were shaken in liquid nitrogen, shearing off trichomes. this procedure yielded a mixture of cells which is highly enriched in trichome. Note that it likely will contain minor contaminations of other types of leaf cells.