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Germinating Seed Library

Library: cLEI
TIGR ID: TGS
Authors: Alcala, Vrebalov, White, and Giovannoni
Date made: 07/99
Species: Lycopersicon esculentum
Accession: TA496
Tissue: whole seedlings
Developmental stage: early germinating seedlings/cotyledons
Vector: pBluescript SK(+/-)
Host: SOLR
Primary pfu: 3.5 x 105
Number mass excised: 2.0 105
Average insert length: 1.4 Kb
Cloning sites: 5' EcoRI, 3' XhoI
Antibiotic: ampicillin
Primers: M13F and M13R
Comments: Seedlings were collected seven days post-imbibition on water-agar. Mixed-stage, whole, germinating seedlings from seed coat emergence up to two centimeters in length were chosen. Seeds not showing obvious signs of germination were discarded. Tissue consists of germinating seedlings at various stages from 2 days imbibition to emergence (approximately four days) to 1 cm (approximately six days) to 2 cm (approximately 8 days). Tissues were harvested based on stage, not day.