0-3 mm flower buds

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Library:	cTOA
TIGR ID:	TFA
Authors:	Rutger van der Hoeven, Julie Bezzerides, and Steve Tanksley
Date made:	09/99
Species:	Lycopersicon esculentum
Accession:	TA496
Tissue:	developing flower buds and/or flowers
Developmental stage:	0-3 mm flower buds from 4-8 week-old plants
Vector:	pBluescript SK(+/-)
Host:	SOLR
Primary pfu:	1.4 x 10⁷
Number mass excised:	1.5 x 10⁶
Average insert length:	1.3 Kb
Cloning sites:	5' EcoRI, 3' XhoI
Antibiotic:	ampicillin
Primers:	M13F and M13R
Comments:	Flower buds and flowers were taken from greenhouse plants (4-8 weeks old, TA496). They were immediately frozen in liquid nitrogen and then size-separated while remaining frozen.

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