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Immature green fruit

Library: cLEM
TIGR ID: TGF
Authors: Alcala, Vrebalov, White, and Giovannoni
Date made: 08/99
Species: Lycopersicon esculentum
Accession: TA496
Tissue: fruit
Developmental stage: mixed fruit 5 dpa - 35 dpa (apx. 5 days prior to mature green)
Vector: pBluescript SK(+/-)
Host: SOLR
Primary pfu: 7.0 x 105
Number mass excised: 2.0 105
Average insert length: 1.6 Kb
Cloning sites: 5' EcoRI, 3' XhoI
Antibiotic: ampicillin
Primers: M13F and M13R
Comments: Fruit were tagged at 5 dpa (0.5 cm) and harvested at 7 day intervals through 35 dpa. Equal masses of tissue from each stage were combined (including seeds and locules) prior to mRNA isolation.