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Breaker fruit

Library: cLEG-R
TIGR ID: TBF
Authors: Alcala, White, and Giovannoni
Date made: 02/00
Species: Lycopersicon esculentum
Accession: TA496
Tissue: fruit pericarp
Developmental stage: breaker
Vector: pBluescript SK(+/-)
Host: SOLR
Primary pfu: 6.6 x 105
Number mass excised: 3.0 105
Average insert length: 1.4 Kb
Cloning sites: 5' EcoRI, 3' XhoI
Antibiotic: ampicillin
Primers: M13F and M13R
Comments: Fruit were harvested at the "breaker" stage (first sign of lycopene accumulation on the blossom end of the fruit). Fruit were cut in half and the seeds and locules were discarded prior to freezing the pericarp.