>SGN-U359588 Coffea canephora #2 [1 ESTs aligned] arabidopsis/peptide: | Symbol: MYB12 | MYB12 belongs to subgroup 7 of the R2R3-MYB family. It strongly activates the promoters of chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), flavonol synthase (FLS) and - to a lesser extent - chalcone flavanone isomerase (CHI), but cannot activate the promoters of flavonoid-3'hydroxylase (F3'H) and dihydroflavonol 4-reductase (DF). The activation requires a functional MYB recognition element (MRE). Results from the myb12-1f allele indicate that an activation domain might be present in the C-terminus. Overexpression or knock-out plants do not show any obvious phenotype under greenhouse conditions. Young myb12-ko seedlings contain reduced amounts of flavonoids (quercetin and kaempferol), while seedlings as well as leaves of MYB12-OX plants displayed an increased flavonoid content. They did not show any significant difference in anthocyanin content. Expression of CHS and FLS shows a clear correlation to MYB12 expression levels. CHI and F3H show increased transcript levels in the MYB12-OX lines, but no differences in the knock-out. Even in the absence of functional MYB12, flavonol biosynthesis is not completely absent, suggesting functional redundancy. | chr2:19483407-19486538 FORWARD | Aliases: T30B22.24, MYB12(evalue: 3e-09, score=58.9) genbank/nr: GHMYB10(evalue: 6e-13, score=77.8)
TTCATGCTTACTTTGATATGTACCTTTTACATTTTGTTGGCATTCTGGCACCGGTAACGA
TACCCTTTTCTGCGTGATGTGTAGCAGATGGGCCAACACGTACTTTTACCTAATTTGTCG
AGAATTTGACAGTTGTTAGGTATAGGCTTTTAGGATTCTTAGACGCTGTATCTTTGAAAG
TCATGGACGTATATAAAATTCTTTCAATTCCCTAAAAATTGGGCTCTATACTCGGGGGAG
AAAGTGTATCATGCTGAAGTGAAATCACAACATCTACAGCATTCCTATGTTCCTAATTCC
TATCTTTTAGTTAAGGGTCATCAGTGTTGCCTTGCAGGTGGTCTTTGATAGCTGGAAGGC
TTCCGGGACGAACAGACAATGAGATCAAGAACTATTGGAACACCAACATCGTCAAAAAAA
TTCAAATTCAAGACAGCCAACATTTAGCCAGCTCCTCCAGCACAAAACGCAAACCGTCAT
CAACTAGGTCCAGATCAGCAGTACTGCAAAATTTAACGAGTGCTGACAAACCACCAAATT
CAGAAAAGCTTGGATCCTTCTGTACTCCTGTGATCCGAACCAAAGCTAGGAGGTGCACTA
AAGTTTTCATCAACGCAGACTTGCAAGCTTCTGCAGGTGCAGAACAAGCCGGTATTATTA
TGGCAGTGAAAGCTCAAGCTCCACCGCCCAGCAGTGATGCCAAAATCAACTTCAACAAAA
CTCCTACTGGTCATAACAACACCGTTGGTCCGGAGCCACCTTCAGGTGGGGTAAAAGAAT
CACTAC
