P A U P * Portable version 4.0b10 for Unix Fri Jun 2 00:50:34 2006 -----------------------------NOTICE----------------------------- This is a beta-test version. Please report any crashes, apparent calculation errors, or other anomalous results. There are no restrictions on publication of results obtained with this version, but you should check the WWW site frequently for bug announcements and/or updated versions. See the README file on the distribution media for details. ---------------------------------------------------------------- Processing of file "~/new_at_family_tcoffee/4_20/cds_nex_4_20/2429.fasta" begins... Warning: Use of all-digit taxon and/or character names does not conform to the NEXUS standard and is strongly discouraged. Remember that names have priority over numbers. Data read in DNA format Data matrix has 7 taxa, 249 characters Valid character-state symbols: ACGT Missing data identified by '?' Gaps identified by '-' "Equate" macros in effect: R,r ==> {AG} Y,y ==> {CT} M,m ==> {AC} K,k ==> {GT} S,s ==> {CG} W,w ==> {AT} H,h ==> {ACT} B,b ==> {CGT} V,v ==> {ACG} D,d ==> {AGT} N,n ==> {ACGT} Processing of file "~/new_at_family_tcoffee/4_20/cds_nex_4_20/2429.fasta" completed. Optimality criterion set to likelihood. Heuristic search settings: Optimality criterion = likelihood Likelihood settings: Number of substitution types = 2 (HKY85 variant) Transition/transversion ratio = 0.9675 (kappa = 1.9738011) Assumed nucleotide frequencies (set by user): A=0.32530 C=0.18200 G=0.22180 T=0.27090 Among-site rate variation: Assumed proportion of invariable sites = none Distribution of rates at variable sites = equal These settings correspond to the HKY85 model Number of distinct data patterns under this model = 99 Molecular clock not enforced Starting branch lengths obtained using Rogers-Swofford approximation method Trees with approximate likelihoods 5% or further from the target score are rejected without additional iteration Branch-length optimization = one-dimensional Newton-Raphson with pass limit=20, delta=1e-06 -ln L (unconstrained) = 1021.04760 Starting tree(s) obtained via stepwise addition Addition sequence: random Number of replicates = 10 Starting seed = 1323435796 Number of trees held at each step during stepwise addition = 1 Branch-swapping algorithm: tree-bisection-reconnection (TBR) Steepest descent option not in effect Initial 'MaxTrees' setting = 100 Branches collapsed (creating polytomies) if branch length is less than or equal to 1e-08 'MulTrees' option in effect Topological constraints not enforced Trees are unrooted Note: Approximation limit dynamically readjusted to 11.039981 Heuristic search completed Total number of rearrangements tried = 144 Score of best tree(s) found = 1159.91927 Number of trees retained = 2 Time used = <1 sec (CPU time = 0.65 sec) Tree-island profile: First Last First Times Island Size tree tree Score replicate hit ---------------------------------------------------------------------- 1 2 1 2 1159.91927 1 10 Tree description: Unrooted tree(s) rooted using outgroup method Optimality criterion = likelihood Likelihood settings: Number of substitution types = 2 (HKY85 variant) Transition/transversion ratio = 0.9675 (kappa = 1.9738011) Assumed nucleotide frequencies (set by user): A=0.32530 C=0.18200 G=0.22180 T=0.27090 Among-site rate variation: Assumed proportion of invariable sites = none Distribution of rates at variable sites = equal These settings correspond to the HKY85 model Number of distinct data patterns under this model = 99 Molecular clock not enforced Starting branch lengths obtained using Rogers-Swofford approximation method Branch-length optimization = one-dimensional Newton-Raphson with pass limit=20, delta=1e-06 -ln L (unconstrained) = 1021.04760 Tree number 1 (rooted using default outgroup) Branch lengths and linkages for tree #1 (unrooted) Connected Branch Standard Node to node length error --------------------------------------------------------- 110565 (1) 11 0.30470 0.04202 10 11 1.36761 0.19141 39028 (2) 10 0 0.01583 9 10 0.02777 0.01232 8 9 0.02624 0.01208 6917 (3) 8 0.00828 0.00591 77118 (4) 8 0.00386 0.00412 At4g39880.1 (5) 9 0.29604 0.04144 AtCg00840 (6) 11 0 undefined AtCg01300 (7) 11 0 undefined --------------------------------------------------------- Sum 2.03451 -Ln likelihood = 1159.91927 /------------ 110565 | | / 39028 | | | | /- 6917 +---------------------------------------------------10/8 11 ||\- 77118 | \9 | \------------ At4g39880.1 | + AtCg00840 | \ AtCg01300 2 trees saved to file "~/new_at_family_tcoffee/4_20/trees_4_20/2429.ml.tre"